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protean ii xi multi cell  (Bio-Rad)


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    Structured Review

    Bio-Rad protean ii xi multi cell
    Protean Ii Xi Multi Cell, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 209 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/protean+ii+xi+multi+cell/PROTEAN+II+xi+Multi-Cell/pm40615700-71-18-23
    Average 93 stars, based on 209 article reviews
    protean ii xi multi cell - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Electrofocusing:

    Article Title: Comparative proteomic analysis of Tunisian barley landraces in response to salinity at the tillering stage.
    Article Snippet: Salinity is one of the major abiotic constraints impairing barley production.. Tunisian landraces constitute a valuable reservoir of useful traits for breeding programs.. Therefore, exploring the molecular mechanisms underlying salinity tolerance is of great interest.

    Article Title: Analysis of DNA methylation alterations in rice seeds induced by different doses of carbon-ion radiation
    Article Snippet: The samples were then applied to 17 cm Immobiline Dry Strips, pH 4–7 (Bio-Rad, Hercules, California, USA). .. Isoelectric focusing (IEF) was performed on a Protean IEF Cell (Bio-Rad) with the following settings: 250 V for 0.5 h, 1000 V for 1 h, 10 000 V for 4 h, 10 000 V for 60 000 Vh and 500 V for 1 h. After IEF, the strips were equilibrated in an equilibration buffer (6 M urea, 30% glycerol, 2% sodium dodecyl sulfate (SDS), a trace of bromophenol blue and 50 mM Tris-HCl; pH 8.8) first with 2% DTT and then without DTT, each for 15 min, and transferred to 12% polyacrylamide gel electrophoresis (SDS-PAGE) gels for 2-DE using a Protean II xi Multi-Cell (Bio-Rad). ..

    Stripping Membranes:

    Article Title: Proteome analysis of Pueraria mirifica tubers collected in different seasons.
    Article Snippet: .. The strip was placed in fresh 1.5 mL equilibration buffer, except with 25 mL/L iodoacetamide (IAA) instead of DTT, for another 20 min and then loaded on the SDS-PAGE (140 g/L acrylamide resolving gel) second dimension (11 × 11 cm) and separated using a PROTEAN II XI multi-cell (BioRad, USA). ..

    SDS Page:

    Article Title: Proteome analysis of Pueraria mirifica tubers collected in different seasons.
    Article Snippet: .. The strip was placed in fresh 1.5 mL equilibration buffer, except with 25 mL/L iodoacetamide (IAA) instead of DTT, for another 20 min and then loaded on the SDS-PAGE (140 g/L acrylamide resolving gel) second dimension (11 × 11 cm) and separated using a PROTEAN II XI multi-cell (BioRad, USA). ..

    Electrophoresis:

    Article Title: Differences in the expression of soluble proteins in freshwater and brackish‐water ecotypes of the snail <scp><i>Theodoxus fluviatilis</i></scp>
    Article Snippet: As a size reference, 10 μl Roti®-Mark STANDARD (Carl Roth, Karlsruhe, Germany) was placed in a small pocket next to the IPG strip. .. The vertical electrophoresis was carried out using a PROTEAN II xi multi-cell (Biorad, Munich, Germany) and started by applying 50 V for 30 min and then increased to 250 V for 4–5 h. Gels were washed in deionized water for 10–15 min (twice) and then transferred to sensitive colloidal Coomassie staining solution (5% w/v aluminum sulfate, 10% v/v ethanol, 0.02 mmol/L CBB G250, 2% v/v orthophosphoric acid) (modified from Kang et al., 2002) overnight. .. The next morning, gels were repeatedly (four times) washed in deionized water for 1–2 h, followed by scanning with an Xfinity Pro42 scanner (Quatographic, Kiel, Germany), and images were saved as tiff files with 300 dpi.

    Staining:

    Article Title: Differences in the expression of soluble proteins in freshwater and brackish‐water ecotypes of the snail <scp><i>Theodoxus fluviatilis</i></scp>
    Article Snippet: As a size reference, 10 μl Roti®-Mark STANDARD (Carl Roth, Karlsruhe, Germany) was placed in a small pocket next to the IPG strip. .. The vertical electrophoresis was carried out using a PROTEAN II xi multi-cell (Biorad, Munich, Germany) and started by applying 50 V for 30 min and then increased to 250 V for 4–5 h. Gels were washed in deionized water for 10–15 min (twice) and then transferred to sensitive colloidal Coomassie staining solution (5% w/v aluminum sulfate, 10% v/v ethanol, 0.02 mmol/L CBB G250, 2% v/v orthophosphoric acid) (modified from Kang et al., 2002) overnight. .. The next morning, gels were repeatedly (four times) washed in deionized water for 1–2 h, followed by scanning with an Xfinity Pro42 scanner (Quatographic, Kiel, Germany), and images were saved as tiff files with 300 dpi.

    Modification:

    Article Title: Differences in the expression of soluble proteins in freshwater and brackish‐water ecotypes of the snail <scp><i>Theodoxus fluviatilis</i></scp>
    Article Snippet: As a size reference, 10 μl Roti®-Mark STANDARD (Carl Roth, Karlsruhe, Germany) was placed in a small pocket next to the IPG strip. .. The vertical electrophoresis was carried out using a PROTEAN II xi multi-cell (Biorad, Munich, Germany) and started by applying 50 V for 30 min and then increased to 250 V for 4–5 h. Gels were washed in deionized water for 10–15 min (twice) and then transferred to sensitive colloidal Coomassie staining solution (5% w/v aluminum sulfate, 10% v/v ethanol, 0.02 mmol/L CBB G250, 2% v/v orthophosphoric acid) (modified from Kang et al., 2002) overnight. .. The next morning, gels were repeatedly (four times) washed in deionized water for 1–2 h, followed by scanning with an Xfinity Pro42 scanner (Quatographic, Kiel, Germany), and images were saved as tiff files with 300 dpi.

    Polyacrylamide Gel Electrophoresis:

    Article Title: Analysis of DNA methylation alterations in rice seeds induced by different doses of carbon-ion radiation
    Article Snippet: The samples were then applied to 17 cm Immobiline Dry Strips, pH 4–7 (Bio-Rad, Hercules, California, USA). .. Isoelectric focusing (IEF) was performed on a Protean IEF Cell (Bio-Rad) with the following settings: 250 V for 0.5 h, 1000 V for 1 h, 10 000 V for 4 h, 10 000 V for 60 000 Vh and 500 V for 1 h. After IEF, the strips were equilibrated in an equilibration buffer (6 M urea, 30% glycerol, 2% sodium dodecyl sulfate (SDS), a trace of bromophenol blue and 50 mM Tris-HCl; pH 8.8) first with 2% DTT and then without DTT, each for 15 min, and transferred to 12% polyacrylamide gel electrophoresis (SDS-PAGE) gels for 2-DE using a Protean II xi Multi-Cell (Bio-Rad). ..



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